Publications
Preprints
Hall, R.N., Weill, U., Khariton, M., Leal-Ortiz, S., Drees, L., Chai, C., Xue, Y., Rosental, B., Quake, S.R., Sánchez Alvarado, A., Melosh, N.A., Fire, A., Rink, J.C., Wang, B.. Heterologous reporter expression in the planarian Schmidtea mediterranea through somatic mRNA transfection. bioRxiv, https://doi.org/10.1101/2021.04.20.440701 (Posted April 20, 2021)
Details
, , , , , Details
Duncan EM, Nowotarski SH, Guerrero-Hernández C, Ross EJ, D’Orazio JA, McKinney SA, Guo L, and Sánchez Alvarado A. A New Species of Planarian Flatworm from Mexico: Girardia Guanajuatiensis. bioRxiv, https://doi.org/10.1101/2020.07.01.183442 (Posted July 02, 2020.) Details
Cultured pluripotent planarian stem cells retain potency and express proteins from exogenously introduced mRNAs.
2021
, , , , , , , Identification of rare, transient post-mitotic cell states that are induced by injury and required for whole-body regeneration in Schmidtea mediterranea
Sonpho E, Mann FG, Levy M, Ross EJ, Guerrero-Hernandez C, Florens L, Saraf A, Doddihal V, Ounjai P, and Sánchez Alvarado A. Decellularization Enables Functional Analysis of ECM Remodeling in Planarian Regeneration. MCP, doi:10.1101/2020.09.11.293936 (Available Online: 18 August 2021 ) Details
Nowotarski SH., Davies E, Robb SMC, Ross EJ, Matentzoglu N, Doddihal V, Mir M, McClain M, and Sánchez Alvarado A. The Planarian Anatomy Ontology: A Resource to Connect Data within and across Experimental Platforms. Development (2021) 148 (15): dev196097. Details
2020
Wang W, Hu SK, Zeng A, Alegre D; Hu D, Gotting K, Ortega Granillo A, Wang Y, Robb S, Schnittker R, Zhang S, Alegre D, Li H, Ross E, Zhang N; Brunet A, Sánchez Alvarado A. 2020. Changes in Regeneration-Responsive Enhancers Shape Regenerative Capacities in Vertebrates. Science 369 (6508): eaaz3090. Details
Sanchez Alvarado A, Stern CD. An interview with Claudio Stern. Int J Dev Biol. 2020 Aug 20. doi: 10.1387/ijdb.190368cs. Epub ahead of print. PMID: 32930375. Details
Kushawah G, Hernandez-Huertas L, Abugattas-Nuñez Del Prado J, Martinez-Morales JR, DeVore ML, Hassan H, Moreno-Sanchez I, Tomas-Gallardo L, Diaz-Moscoso A, Monges DE, Guelfo JR, Theune WC, Brannan EO, Wang W, Corbin, TJ, Moran AM, Sánchez Alvarado A, Málaga-Trillo E, Takacs CM, Bazzini AA, Moreno-Mateos MA, 2020. CRISPR-Cas13d Induces Efficient mRNA Knockdown in Animal Embryos. Dev. Cell. https://doi.org/10.1016/j.devcel.2020.07.013 Details
Sánchez Alvarado A. Model system might reveal how coral cells take up and evict algae. Nature. 2020 Jun;582(7813):495-496. doi: 10.1038/d41586-020-01715-9. PMID: 32572250. Details
Hu CK, Wang W, Brind’Amour J, Singh PP, Reeves GA, Lorincz MC, Sánchez Alvarado A, Brunet A. Vertebrate diapause preserves organisms long term through Polycomb complex members. Science. 2020 Feb 21;367(6480):870-874. doi: 10.1126/science.aaw2601. PubMed PMID: 32079766. Details
2019
Kim IV, Duncan EM, Ross EJ, Gorbovytska V, Nowotarski SH, Elliott SA, Sánchez Alvarado A, Kuhn CD. Planarians recruit piRNAs for mRNA turnover in adult stem cells. Genes Dev. 2019 Nov 1;33(21-22):1575-1590. doi: 10.1101/gad.322776.118. Epub 2019 Sep 19. Details
Duncan EM, Sánchez Alvarado A. Regulation of Genomic Output and (Pluri)potency in Regeneration. Annual Review of Genetics. 2019 Dec 3;53:327-346. doi: 10.1146/annurev-genet-112618-043733. Epub 2019 Sep 10. Details
Arnold CP, Benham-Pyle BW, Lange JJ, Wood, CJ, Sánchez Alvarado A. Wnt and TGFβ coordinate growth and patterning to regulate size-dependent behaviour. 2019. Nature. doi:10.1038/s41586-019-1478-7. Details
Sun J, Mu H, Ip JCH, Li R, Xu T, Accorsi A, Sánchez Alvarado A, Ross E, Lan Y, Sun Y, Castro-Vazquez A, Vega IA, Heras H, Ituarte S, Van Bocxlaer B, Hayes KA, Cowie RH, Zhao Z, Zhang Y, Qian PY, Qiu JW. Signatures of Divergence, Invasiveness, and Terrestrialization Revealed by Four Apple Snail Genomes. Molecular Biology and Evolution. 2019 Jul 1;36(7):1507-1520. Details
2018
Cheng LC, Sánchez Alvarado A. Whole-Mount BrdU Staining with Fluorescence In Situ Hybridization in Planarians. Methods in Molecular Biology. 2018;1774:423—434. Details
Merryman MS, Sánchez Alvarado A, Jenkin JC. Culturing Planarians in the Laboratory. Methods in Molecular Biology. 2018;1774:241—258. Details
Zhang S, Sánchez Alvarado A. Planarian High Molecular Weight DNA Isolation by Spooling. Methods in Molecular Biology. 2018;1774:277—284. Details
Adler CE, Sánchez Alvarado A. Systemic RNA Interference in Planarians by Feeding of dsRNA Containing Bacteria. Methods in Molecular Biology. 2018;1774:445—454. Details
Zeng A, Li H, Guo L, Gao X, McKinney S, Wang Y, Yu Z, Park J, Semerad C, Ross E, Cheng LC, Davies E, Lei K,Wang W, Perera A, Hall K, Peak A, Box A, Sánchez Alvarado A. Prospectively Isolated Tetraspanin+ Neoblasts Are Adult Pluripotent Stem Cells Underlying Planaria Regeneration. Cell. 2018 Jun;173(7):1593—1608.e20. Details
Guo L, Accorsi A, He S, Guerrero-Hernández C, Sivagnanam S, McKinney S, Gibson M, Sánchez Alvarado A. An adaptable chromosome preparation methodology for use in invertebrate research organisms. BMC biology. 2018 Feb;16(1):25. Details
Cheng LC, Tu KC, Seidel CW, Robb SMC, Guo F, Sánchez Alvarado A. Cellular, ultrastructural and molecular analyses of epidermal cell development in the planarian Schmidtea mediterranea. Developmental Biology. 2018 Jan;433(2):357—373. Details
Sánchez Alvarado A. To solve old problems, study new research organisms. Developmental Biology. 2018 Jan;433(2):111—114. Details
2017
Accorsi A, Williams M, Ross EJ, Robb SMC, Elliott SA, Tu KC, Sánchez Alvarado A Hands-On Classroom Activities for Exploring Regeneration and Stem Cell Biology with Planarians The American Biology Teacher, Vol. 79 No. 3, March 2017; (pp. 208-223) DOI: 10.1525/abt.2017.79.3.208 Details
Sasidharan V, Marepally S, Elliott SA, Baid S, Lakshmanan V, Nayyar N, Bansal D, Sánchez Alvarado A, Vemula PK, Palakodeti D. The miR-124 family of microRNAs is crucial for regeneration of the brain and visual system in the planarian Schmidtea mediterranea. Development (Cambridge, England). 2017 Sep;144(18):3211—3223. Details
Adler CE, Sánchez Alvarado A. PHRED-1 is a divergent neurexin-1 homolog that organizes muscle fibers and patterns organs during regeneration. Developmental Biology. 2017 Jul;427(1):165—175. Details
Davies EL, Lei K, Seidel CW, Kroesen AE, McKinney SA, Guo L, Robb SM, Ross EJ, Gotting K, Sánchez Alvarado A. Embryonic origin of adult stem cells required for tissue homeostasis and regeneration. Elife. 2017 Jan 10. Details
2016
Sánchez Alvarado, A. The joy of figuring things out: a story of worms, haplotypes & genetic ancestry. Dec 05, 2016. https://natureecoevocommunity.nature.com/users/23284-alejandro-sanchez-alvarado/posts/13784-worms-genetics-haplotypes-ancestry Details
Nowotarski SH, Sánchez Alvarado A. Widening perspectives on regenerative processes through growth. NPJ Regenerative Medicine (2016) 1, 16015; Details
Guo L, Zhang S, Rubinstein B, Ross E, Sánchez Alvarado A. Widespread maintenance of genome heterozygosity in Schmidtea mediterranea. Nature Ecology & Evolution. 2016 Dec;1(1):19. Details
Luttrell SM, Gotting K, Ross E, Sánchez Alvarado A, Swalla BJ. Head regeneration in hemichordates is not a strict recapitulation of development. Developmental Dynamics. 2016 Dec;245(12):1159—1175. Details
Lei K, Thi-Kim Vu H, Mohan RD, McKinney SA, Seidel CW, Alexander R, Gotting K, Workman JL, Sánchez Alvarado A. Egf Signaling Directs Neoblast Repopulation by Regulating Asymmetric Cell Division in Planarians. Developmental Cell. 2016 Aug;38(4):413—429. Details
Arnold CP, Merryman MS, Harris-Arnold A, McKinney SA, Seidel CW, Loethen S, Proctor KN, Guo L, Sánchez Alvarado A. Pathogenic shifts in endogenous microbiota impede tissue regeneration via distinct activation of TAK1/MKK/p38. eLife. 2016 Jul 21. Details
Ross E, Blair D, Guerrero-Hernández C, Sánchez Alvarado A. Comparative and Transcriptome Analyses Uncover Key Aspects of Coding- and Long Noncoding RNAs in Flatworm Mitochondrial Genomes. G3. 2016 May;6(5):1191—1200. Details
2015
Duncan EM, Chitsazan AD, Seidel CW, Alvarado AS. Set1 and MLL1/2 Target Distinct Sets of Functionally Different Genomic Loci In Vivo. Cell Reports. 2016 Oct;17(3):930 Details
Tu KC, Cheng LC, T K Vu H, Lange JJ, McKinney SA, Seidel CW, Sánchez Alvarado A. Egr-5 is a post-mitotic regulator of planarian epidermal differentiation. eLife. 2015 Oct 12 Details
Adler CE, Sánchez Alvarado A. Types or States? Cellular Dynamics and Regenerative Potential. Trends in Cell Biology. 2015 Nov;25(11):687—696. Details
Robb SMC, Gotting K, Ross E, Sánchez Alvarado A. SmedGD 2.0: The Schmidtea mediterranea genome database. Genesis. 2015 Aug;53(8):535—546. Details
Thi-Kim Vu H, Rink JC, McKinney SA, McClain M, Lakshmanaperumal N, Alexander R, Sánchez Alvarado, A. Stem cells and fluid flow drive cyst formation in an invertebrate excretory organ. eLife. 2015 Jun
Details
Sánchez Alvarado, A. Unravelling a can of worms. eLife. 2015
Details2014
Xiang Y, Miller DE, Ross EJ, Sánchez Alvarado A, Hawley RS. Synaptonemal complex extension from clustered telomeres mediates full-length chromosome pairing in Schmidtea mediterranea. PNAS. 2014 Dec;111(48):E5159—68. Details
Adler CE, Seidel CW, McKinney SA, Sánchez Alvarado A. Selective amputation of the pharynx identifies a FoxA-dependent regeneration program in planaria. eLife. 2014 Apr;3:e02238. (See editorial Insight by Dr. Janet Rossant) Details
Sánchez Alvarado A, Yamanaka S. Rethinking differentiation: stem cells, regeneration, and plasticity. Cell. 2014 Mar;157(1):110—119. Details
Robb SMC, Sánchez Alvarado A. Histone modifications and regeneration in the planarian Schmidtea mediterranea. Current Topics in Developmental Biology. 2014;108:71—93. Details
2013
Rossi A, Ross EJ, Jack A, Sánchez Alvarado A. Molecular cloning and characterization of SL3: a stem cell-specific SL RNA from the planarian Schmidtea mediterranea. Gene. 2014 Jan;533(1):156—167. Details
Sánchez Alvarado A. Learning about loss. eLife. 2013;2:e00533. Details
Sánchez Alvarado A. On the trail of a tropical disease. eLife. 2013;2:e01115. Details
2012
Sánchez Alvarado A. Q&A: what is regeneration, and why look to planarians for answers? BMC Biology. 2012 Nov;10:88. Details
Sánchez Alvarado A. Cellular hyperproliferation and cancer as evolutionary variables. Current Biology. 2012 Sep;22(17):R772—8. Details
Guedelhoefer OC, Sánchez Alvarado A. Amputation induces stem cell mobilization to sites of injury during planarian regeneration. Development. 2012 Oct;139(19):3510—3520. Details
Guedelhoefer OC, Sánchez Alvarado A. Planarian immobilization, partial irradiation, and tissue transplantation Journal of Visualized Experiments (JoVE), 2012 August 5 Details
Elliott SA, Sánchez Alvarado A. The history and enduring contributions of planarians to the study of animal regeneration. WIREs Developmental Biology. 2013;2(3):301—326. Details
Tu KC, Pearson BJ, Sánchez Alvarado A. TORC1 is required to balance cell proliferation and cell death in planarians. Developmental Biology. 2012 May;365(2):458—469. Details
Bender CE, Fitzgerald P, Tait SWG, Llambi F, McStay GP, Tupper DO, Pellettieri J, Sánchez Alvarado A, Salvesena GS, Green DR. Mitochondrial pathway of apoptosis is ancestral in metazoans. PNAS. 2012 Mar;109(13):4904—4909. Details
Azimzadeh J, Wong ML, Downhour DM, Sánchez Alvarado A, Marshall WF. Centrosome loss in the evolution of planarians. Science (New York, NY). 2012 Jan;335(6067):461—463. Details
2011
Rink JC, Thi-Kim Vu H, Sánchez Alvarado A. The maintenance and regeneration of the planarian excretory system are regulated by EGFR signaling. Development. 2011 Sep;138(17):3769—3780. Details
Adamidi C, Wang Y, Gruen D, Mastrobuoni G, You X, Tolle D, Dodt M, Mackowiak SD, Gogol-Doering A, Oenal P, Rybak A, Ross E, Sánchez Alvarado A, Kempa S, Dieterich C, Rajewsky N, Chen W. De novo assembly and validation of planaria transcriptome by massive parallel sequencing and shotgun proteomics. Genome Research. 2011 Jul;21(7):1193—1200. Details
2010
Gurley KA, Elliott SA, Simakov O, Schmidt HA, Holstein TW, Sánchez Alvarado A. Expression of secreted Wnt pathway components reveals unexpected complexity of the planarian amputation response. Developmental Biology. 2010 Nov;347(1):24—39. Details
2009
Pearson BJ, Sánchez Alvarado A. A planarian p53 homolog regulates proliferation and self-renewal in adult stem cell lineages. Development. 2010 Jan;137(2):213—221. Details
Rink JC, Gurley KA, Elliott SA, Sánchez Alvarado A. Planarian Hh signaling regulates regeneration polarity and links Hh pathway evolution to cilia. Science. 2009 Dec;326(5958):1406—1410. Details
Pellettieri J, Fitzgerald P, Watanabe S, Mancuso J, Green DR, Sánchez Alvarado A. Cell death and tissue remodeling in planarian regeneration. Developmental Biology. 2010 Feb;338(1):76—85. Details
Sánchez Alvarado A. A cellular view of regeneration. Nature. 2009 Jul;460(7251):39—40. Details
Friedländer MR, Adamidi C, Han T, Lebedeva S, Isenbarger TA, Hirst M, Marra M, Nusbaum C, Lee WL, Jenkin JC, Sánchez Alvarado A, Kim JK, Rajewsky N. High-resolution profiling and discovery of planarian small RNAs. PNAS. 2009 Jul;106(28):11546—11551. Details
Kang H, Sánchez Alvarado A. Flow cytometry methods for the study of cell-cycle parameters of planarian stem cells. Developmental Dynamics. 2009 May;238(5):1111—1117. Details
Pearson BJ, Eisenhoffer GT, Gurley KA, Rink JC, Miller DE, Sánchez Alvarado A. Formaldehyde-based whole-mount in situ hybridization method for planarians. Developmental Dynamics. 2009 Feb;238(2):443—450. Details
Pearson BJ, Sánchez Alvarado A. Regeneration, Stem Cells, and the Evolution of Tumor Suppression Cold Spring Harb Symp Quant Biol. 2009 Jan 15. Details
2008
Sánchez Alvarado A. The Shredding of a Caricature Cell, 135:991-992, December 12, 2008 Details
Gurley KA, Sánchez Alvarado A. Stem Cells in Animal Models of Regeneration StemBook, ed. The Stem Cell Research Community, StemBook, Details
Oviedo NJ, Pearson BJ, Levin M, Sánchez Alvarado A. Planarian PTEN homologs regulate stem cells and regeneration through TOR signaling. Disease Models & Mechanisms. 2008;1(2–3):131—43. Details
Eisenhoffer GT, Kang H, Sánchez Alvarado A. Molecular analysis of stem cells and their descendants during cell turnover and regeneration in the planarian Schmidtea mediterranea. Cell stem cell. 2008 Sep;3(3):327—339. Details
Sánchez Alvarado A. Stem Cells: Time to Check Our Premises. Cell Stem Cell, 3:25-9. Details
Reddien PW, Newmark PA, Sánchez Alvarado A. Gene nomenclature guidelines for the planarian Schmidtea mediterranea. Developmental Dynamics. 2008 Nov;237(11):3099—3101. Details
Birnbaum KD, Sánchez Alvarado A. Slicing across kingdoms: regeneration in plants and animals. Cell. 2008 Feb;132(4):697—710. Details
2007
Cantarel BL, Korf I, Robb SMC, Parra G, Ross E, Moore B, Holt C, Sánchez Alvarado A, Yandell M. MAKER: an easy-to-use annotation pipeline designed for emerging model organism genomes. Genome Research. 2008 Jan;18(1):188—196. Details
Pellettieri J, Sánchez Alvarado A. Cell turnover and adult tissue homeostasis: from humans to planarians. Annual Review of Genetics. 2007;41:83—105. Details
Robb SMC, Ross E, Sánchez Alvarado A. SmedGD: the Schmidtea mediterranea genome database. Nucleic Acids Research. 2008 Jan;36(Database issue):D599—606. Details
Reddien PW, Bermange AL, Kicza AM, Sánchez Alvarado A. BMP signaling regulates the dorsal planarian midline and is needed for asymmetric regeneration. Development. 2007 Nov;134(22):4043—4051. Details
Gurley KA, Rink JC, Sánchez Alvarado A. Beta-catenin defines head versus tail identity during planarian regeneration and homeostasis. Science. 2008 Jan;319(5861):323—327. Details
2006
Sánchez Alvarado A, Tsonis PA. Bridging the regeneration gap: genetic insights from diverse animal models. Nature Reviews Genetics. 2006 Nov;7(11):873—884. Details
Spradling A, Ganetsky B, Hieter P, Johnston M, Olson M, Orr-Weaver T, Rossant J, Sánchez Alvarado A, Waterston R. New Roles for Model Genetic Organisms in Understanding and Treating Human Disease: Report From The 2006 Genetics Society of America Meeting. Genetics 172(4): 2025-2032. Details
Orendt A, Haymore, Richardson D, Robb SMC, Sánchez Alvarado A, Facelli JC. Design, Implementation and Deployment of a Commodity Cluster for Periodic Comparisons of Gene Sequences, in High-Performance Computing Paradigm and Infrastructure. 2006 M.G. Laurence, T. Yang, Editor. 733-744. Details
Sánchez Alvarado A. Planarian regeneration: its end is its beginning. Cell. 2006 Jan;124(2):241—245. Details
2005
Reddien PW, Oviedo NJ, Jennings JR, Jenkin JC, Sánchez Alvarado A. SMEDWI-2 is a PIWI-like protein that regulates planarian stem cells. Science (New York, NY). 2005 Nov;310(5752):1327—1330. Details
Sánchez Alvarado A, Kang H. Multicellularity, stem cells, and the neoblasts of the planarian Schmidtea mediterranea. Experimental cell research. 2005 Jun;306(2):299—308. Details
Reddien PW, Bermange AL, Murfitt KJ, Jennings JR, Sánchez Alvarado A. Identification of genes needed for regeneration, stem cell function, and tissue homeostasis by systematic gene perturbation in planaria. Developmental Cell. 2005 May;8(5):635—649. Details
2004
Reddien PW, Sánchez Alvarado A. Fundamentals of Planarian Regeneration. Annual Review of Cell and Developmental Biology. 2004;20:725—757. Details
Sánchez Alvarado A. Planarians. Current biology : CB. 2004 Sep;14(18):R737—8. Details
Alejandro Sánchez Alvarado (2004) Regeneration and the need for simpler model organisms Philosophical Transactions of the Royal Society of London. Series B 359: 759-763. 
Details
DetailsInoue T, Kumamoto H, Okamoto K, Umesono Y, Sakai M, Sánchez Alvarado A, Agata K. Morphological and functional recovery of the planarian photosensing system during head regeneration. Zoological Science. 2004 Mar;21(3):275—283. Details
2003
Newmark PA, Reddien PW, Cebrià F, Sánchez Alvarado A. Ingestion of bacterially expressed double-stranded RNA inhibits gene expression in planarians. PNAS. 2003 Sep;100 Suppl 1:11861—11865. Details
Sánchez Alvarado A. The freshwater planarian Schmidtea mediterranea: embryogenesis, stem cells and regeneration. Current Opinion in Genetics & Development. 2003 Aug;13(4):438—444. Details
Oviedo NJ, Newmark PA, Sánchez Alvarado A. Allometric scaling and proportion regulation in the freshwater planarian Schmidtea mediterranea. Developmental Dynamics. 2003 Feb;226(2):326—333. Details
2002
Sánchez Alvarado A, Newmark PA, Robb SM, Juste R. The Schmidtea mediterranea database as a molecular resource for studying platyhelminthes, stem cells and regeneration. Development . 2002 Dec;129(24):5659—5665. Details
Cebrià F, Kobayashi C, Umesono Y, Nakazawa M, Mineta K, Ikeo K, Gojobori T, Itohk M, Tairak M, Sánchez Alvarado A, Agata K. FGFR-related gene nou-darake restricts brain tissues to the head region of planarians. Nature. 2002 Oct;419(6907):620—624. Details
Newmark PA, Sánchez Alvarado A. Not your father’s planarian: a classic model enters the era of functional genomics. Nature Reviews Genetics. 2002 Mar;3(3):210—219. Details
Robb SM, Sánchez Alvarado A. Identification of immunological reagents for use in the study of freshwater planarians by means of whole-mount immunofluorescence and confocal microscopy. Genesis. 2002 Apr;32(4):293—298. Details
2000
Sánchez Alvarado A. Regeneration in the Metazoans: Why Does it Happen? 2000. BioEssays. 22:578-590. Details
Newmark PA, Sánchez Alvarado A. Bromodeoxyuridine Specifically Labels the Regenerative Stem Cells of Planarians. 2000. Developmental Biology. 220:142-153. Details
Sánchez Alvarado A. The Case for Comparative Regeneration: Learning from Simpler Organisms How to Make New Parts from Old 2000. e-biomed: The Journal of Regenerative Medicine. 1: 31-36. Details
1999
Sánchez Alvarado A, Newmark P. Double-stranded RNA specifically disrupts gene expression during planarian regeneration. PNAS. 1999 Apr;96(9):5049—5054. Details
Sánchez Alvarado A, Invited Article: “La Nueva Biologia” El Universal, Caracas, Venezuela, February 13, 1999. Details
Newmark PA, Sánchez Alvarado A. Planarian Regeneration. 1999. Embryonic Encyclopedia of Life Sciences. Nature Publishing Group, London. Details
Sánchez Alvarado A, Invited Article: Ciencia y Mitología de la Regeneración Animal. El Universal, Caracas, Venezuela, September 12, 1998. Details
1998
Sánchez Alvarado A, Newmark PA. The Use of Planarians to Dissect the Molecular Basis of Metazoan Regeneration. 1998. Wound Repair and Regeneration. 6: 413-420. Details
